All Articles

Use of Microfluidic Assays to Develop Reliable and Economic Nucleic Acid Application Technologies, Employing MicroRNAs for the Diagnostic Screening of Colon Cancer in Human Stool in Low-Resource Settings

Abstract:

Isolation methods that employ readily-available inexpensive supplies on the open market, which are reliable, as well as economical, such as nucleic acid amplification techniques (NAAT) based on microfluidic technology in low-resource research settings (LRRS) that meets the ASSURED guidelines are essential to develop a noninvasive diagnostic colon cancer screen in stool using micro(mi)RNA molecules. A combination of a microfluidic-based MiRNA stool test with a reliable rolling circle amplification/detection method applied to the quantification of miRNA molecules, result in an affordable sensitive and specific isothermal method for the noninvasive quantitative detection of miRNAs in LRRS.

Scientists and engineers have become interested in miRNAs, and they have intensified their efforts to apply emerging simple detection tools to the important bioanalytical challenge of quantifying these small 18-26 nt long molecules. Some of the proposed approaches i...

Effects of Aluminium Sulphates on Weight and Structure of Adrenal Gland of Adult Wistar Rats

Abstract:

This study evaluated the effects of aluminum sulphate exposure on the histology of adrenal gland of Wistar rats. Thirty adult Wistar rats were used for this study. The Wister rats were divided into three groups; group A was the control animals and tagged C, group B animals received 10g of alum dissolved in 1000cm3 of distilled water and were tagged T1, group C animals received 50g of alum dissolved in 1000cm3of distilled water and were tagged T2, via drinking water for duration of four weeks. Twenty-four hours after the last administration, the rats were sacrificed by cervical dislocation. The adrenal gland was excised and preserved in 10 % formosaline after which it was routinely processed for hematoxylin and eosin staining (H&E).

Histological observations showed normal cell distribution in the control group but treated group revealed evidences o...

Is the Onion Router a Secure Network?

Abstract:

This paper attempts to answer the question of how government agencies use The Onion Router, or Tor, in conducting investigations. The essay observes that Tor is free open-source software that can be used by anyone who desires to communicate with others in a secure manner. In surveying the literature, it is found that Tor can be employed for both legal and illegal purposes. As the major financial contributor, Tor is used by government to secure its communications. Because Tor by bad actors, and because many of the Tor relays are operated by government agencies, Tor has been successfully employed in catching unsuspecting criminals. The conclusion of this exposition is that if one wants to communicate securely with someone else, and not be visible to government agencies, Tor should not be the vehicle of choice. There are alternative modes of communication that can thwart the risk of government surveillance.

Brazil and COVID-19: Challenges of Remote Education in Basic Education

Abstract:

A commentary on remote education challenges during COVID‑19 in Brazil. It covers equity, infrastructure, teacher training, and student engagement, proposing pragmatic steps to support learning continuity.

Bacteriological Quality of Groundwater in Imiringi Town, Bayelsa State, Nigeria

Abstract:

This study evaluated the bacteriological quality of groundwater from Imiringi in Ogbia Local government area of Bayelsa State, Nigeria. Groundwater samples were obtained in triplicates from four locations in the study area and analyzed for the bacteria quality using standard protocols. Results showed that the microbial density ranged from 2.97 – 6.03 x 10⁴ cfu/ml (for total heterotrophic bacteria counts), 4.17 – 5.43 x 10² cfu/ml (for Enterobacteriaceae bacteria counts) and 0.00 – 1.48 x 10² cfu/ml (for Salmonella-Shigella counts). Statistically, there was no significant difference (p>0.05) across the various locations for each of the parameters. The density observed were higher than the allowable limit of 1.0 x 10² cfu/ml for drinking water as recommended by World Health Organization/Food and Agricultural Organization, and maximum permissible level of 10cfu/ml for total coliform as specified by...

Laboratory Tests Used in the Diagnosis of Immune Thrombocytopenia and General Treatment Approaches

Abstract:

Immune thrombocytopenia currently called under its’ new name, immune thrombocytopenic purpura (ITP) is a disease characterized by thrombocytopenia, in which the body attacks its own platelets due to the disorders in immune system. The pathophysiology of this disease includes increased platelet destruction and most megakaryocyte production in bone marrow. The most common clinical manifestation of ITP is mild or severe progressive bleeding that could result in death. ITP is generally named as primary or secondary ITP according to thrombocytopenia severity, disease duration, bleeding status and secondary occurrence of the disease. Currently for diagnosis, despite the blood count, antiglobulin test and laboratory tests that can detect platelet-bound antibodies, they are not enough for definitive diagnosis. Like the difficulty in diagnosis, ITP treatment is quite complicated which varies depending on age, characteristics and risk of the patient. It is classified as f...

Therapeutic Evaluation of Neemazal® Against Experimental Eimeria Tenella Infection in Broiler Chickens, Jos - Nigeria

Abstract:

Coccidiosis in poultry is caused by protozoan parasites of the Eimeria species, which is responsible for worldwide economic losses. The aim of this study was to evaluated the therapeutic effect of NeemAzal® on Eimeria tenellain broiler Chickens as compared to Amprolium as a standard anticoccidial drug. A total of One Hundred and Sixty (160) broiler chicks were purchased, acclimatized and randomly divided into 4 groups (G1, G2, G3 & G4). G1 non-infected, non-treated (negative control), (G2) infected with 20000 E. tenella oocysts (positive control), (G3) infected and treated with Amprolium (Standard, 7 mg/kg b.w. for 5 days) and (G4) infected and treated with NeemAzal®200 mg/kg b.w. for 5 days). Evaluation was by clinical signs, performance data (weight gain, oocyst shed/gram faeces (OPG) and histopathology of the Caecum, Liver and Kidney. The data showed that birds infected w...

Molecular Study of Hepcidin HAMP (-582A/G) Gene Polymorphisms and Measurement of Serum Hepcidin Level among Sudanese Patients with Anemia of Chronic Kidney Disease

Abstract:

Anemia of chronic disease is anemia found in certain chronic disease states, is typically marked by the disturbance of iron homeostasis or hypoferremia. Chronic renal failure is currently known as Chronic Kidney Disease (CKD) or Chronic Renal Insufficiency (CRI) implies long-standing, progressive and irreversible renal parenchyma disease resulting in diminished renal function up to 40 to 60%. Often, chronic kidney disease is diagnosed as a result of screening of people known to be at risk of kidney problems, such as those with high blood pressure or diabetes and those with a blood relative with chronic kidney disease. This disease may also be identified when it leads to one of its recognized complications such as cardiovascular disease, anemia, or pericarditis.                            

How Valid are the Reported Cases of People Infected with Covid-19 in the World?

Abstract:

The goal of this paper is to analyze the registered cases of people who have been infected with Covid-19 registered from throughout the world, using a digital forensic analysis technique that is based on Benford's Law. Twenty-three countries were randomly chosen for this analysis: China, India, Germany, Brazil, Venezuela, Netherlands, Italy, Colombia, Russia, Norway, South Africa, Portugal, Singapore, United Kingdom, Chile, Ecuador, Egypt, Denmark, Ireland, France, Belgium, Australia and Croatia.. We calculate on the p-values based on Pearson χ² and Mantissa Arc Test according to the results obtained with the first digit. If any country fails these two tests, a third proof will be carried out based on the Freedman-Watson test. The results indicated that results from Italy, Portugal, Netherlands, United Kingdom, Denmark, Belgium and Chile are suspicions of data manipulation because the numbers fail the Benford’s Law according to the results obtained u...

Should an Online Blogger Be Protected by the Illinois Reporter’s Privilege?

Abstract:

The question that this paper answer is whether the Illinois Reporter’s Privilege should protect an online blogger. The definitions of the terms “reporter,” “news medium,” and “source” quoted from 735 ILCS 5/8-902, followed by several examples. Second, a brief history of journalism is presented, where it is demonstrated that for hundreds of years, journalists and publishers alike engaged in their profession with little formal training, but rather with a sincere desire to convey the facts and the truth to their peers. Second, the essay outlines how Illinois and federal courts have employed the privilege in case law. In answering the question, the work examines whether WikiLeaks qualifies under the Illinois Reporter’s Privilege. The position taken is that the answer is yes. The idea is that if WikiLeaks qualifies under the privilege, then other online blogs also qualify. The opinion of the author is that the law is sufficient as it stands. There is no...