Effects of Glutamine and Glycine Supplementation on Invitro Vitrification of Immature bovine oocytes
The present study was designed to investigate the effects of supplementation of the maturation media with glutamine and glycine onthe invitro maturation of immature oocytes. Different concentration of glutamine and glycine (0.5, 1, 2 mM) were used in the current study. The oocytes morphology, viability, cleavage, and blastocyst rates were the dependent variables in the present study (p<0.05). The highest morphology score was reported when using when1 mM glutamine and glycine compared to control (p<0.05). Addition of 0.5Mm glutamine and glycine not significantly differ from control group. Moreover, addition of 2Mm glutamine and glycine significantly decreased normal morphology % when compared to control group. The highest viability rate was reported when using 1mM of glutamine and glycine (p<0.05) which is significantly different than control and 0.5mM. Addition of 2mM significantly decreased viability when compared to control group. The highest cleavage rate was reported when using 1mM glutamine and glycine which was significantly increased when compared to control group (p<0.05). Addition of 0.5mM glutamine and glycine significantly increased cleavage rate when compared to control group (p<0.05). Addition of 2mMglutamine and glycine significantly decreased cleavage rate when compared to control group. The highest blastocyst rate was reported when using 1mM glutamine and glycine which significantly increased blastocyst formation rate when compared to control. Addition of 0.5mM glutamine and glycine significantly increased this parameter when compared to control group but addition of 2mM result didn’t differ from control (p>0.05). The highest morphology score, viability rate, cleavage rate and blastocyst formation rate were reported when using 1mM glutamine and glycine.