Marwa EL-Adl, Wael Eldomany, Abdel Rouf Hegab, Ahmed Monir, Mohammed A Elmetwally, Investigating the Effect of Dopamine on in Vitro Maturation, Fertilization and Development of Immature Bovine Oocytes, Journal of Veterinary Healthcare, Volume 3, Issue 2, 2024, Pages 01-15, ISSN 2575-1212, https://doi.org/10.14302/issn.2575-1212.jvhc-24-4888.
(https://oap-onlinejournals.org/veterinary-healthcare/article/investigating-the-effect-of-dopamine-on-in-vitro-maturation-fertilization-and-development-of-immature-bovine-oocytes-2374)
Abstract: Proteins and other nitrogenous substances such as polyamines, catecholamines, and nitric oxide require amino acids for synthesis. The purpose of this study was to see how adding dopamine to either maturation or fertilization medium affected the developmental competence of immature bovine oocytes. In this study, Ovaries from apparently normal reproductive organs of cattle were collected within 30 minutes from slaughter and evisceration of animals. Cumulus oocyte complexes (COCs) were collected by aspiration of medium sized ovarian follicles (4-8 mm). COCs of acceptable quality were selected, washed and incubated in tissue culture media (TCM) 199 supplemented with 10% heat inactivated fetal calf serum, 5 μg/ml luteinizing hormone, 0.5 μg/ ml follicle stimulating hormone and 1 μg/ml estradiol-17β for 20:22 hour at 38.5 C◦ under 5% CO2 in air with 90% humidity. different concentrations of dopamine (20,40 ng) were used. The results were consistent for both maturation and fertilization and There is non-significant increase in maturation and fertilization.  
Keywords: bovine; dopamine; maturation; fertilization